Fig. 2
From: Defective Slc7a7 transport reduces erythropoietin compromising erythropoiesis
Slc7a7 intrinsic expression does not drive deficient erythropoiesis in Slc7a7 knockout mice. a Colony-forming unit assays of 12,500 Lin−Sca-1+c-Kit+ cells isolated from control and Slc7a7KO mice. The colony types included granulocyte/monocyte (G/M/GM), granulocyte (G), monocyte (M), megakaryocyte/erythroid (Mk/E), and mixed granulocyte-erythroid-monocyte-megakaryocyte (GEMM) CFUs (n = 4). b Real-time PCR analysis of Slc7a7 expression in sorted erythroid progenitors (highlighting stage-specific expression I–IV) from control mice. c Real-time PCR analysis of Slc7a7 expression across erythroid progenitor stages (IV, III, II, I) in control and Slc7a7KO mice. d Schematic representation of bone marrow transplantation in control (CD45.1) and Slc7a7KO (CD45.2) mice. The experimental setup involves transplantation of wild-type cells into Slc7a7KO recipients and vice versa, followed by tamoxifen and a low-protein diet. e Left: Representative dot plots showing the gating strategy for erythroid progenitors in bone marrow, identifying five clusters (I–V). Right: Quantification of erythroid progenitor populations. Clusters I–III showed no significant differences, whereas reductions were observed in Cluster II (P = 0.0027) and Cluster V (P < 0.0001) in Slc7a7KO mice. Representative data from three independent experiments are shown. Statistical analysis was performed via two-way ANOVA. f Complete blood count analysis of the transplanted mice. Slc7a7KO recipients transplanted with wild-type cells (filled circles) and wild-type recipients transplanted with Slc7a7KO cells (empty circles) presented significant differences in the mean corpuscular volume (MCV, P = 0.027) but no significant changes in HGB, RBC, or MCH. j Schematic representation of Slc7a7-specific ablation in myeloid cells via Lyz2Cre (Slc7a7LysM) and erythroid cells via ErGFPCre (Slc7a7EpoR). k Quantification of erythroid precursor populations (Stages I–V) in Slc7a7LysMKO, Slc7a7EpoRKO, and their respective control mice. The data are from n = 7 (Lyz2Cre) and n = 3 (EpoR) mice. Statistical analysis was performed via two-way ANOVA. Unless specified, all the data are presented as the means ± SEMs. Statistical analysis was performed via a two-tailed unpaired Student’s t test. Each data point represents a single animal