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Fig. 8 | Molecular Medicine

Fig. 8

From: BACH1 deficiency improves placental angiogenesis via SLC25A51-mediated mitochondrial NAD+ transport in intrahepatic cholestasis of pregnancy

Fig. 8

BAHC1 inhibitor HPPE promotes TCA-induced HUVECs proliferation. A Viability of HUVECs treated with different concentrations of HPPE (0, 1, 2.5, 5, 10, and 20 μM; n = 3). B Western blotting results for BACH1 in HUVECs treated with different concentrations of HPPE (0, 1, 2.5, 5, 10, and 20 μM); bar graph represents quantification of gray value (n = 3). C Western blotting results for SLC25 A51, VEGFR2, CD146, CD34, CD105, vWF, VEGFA, and PCNA in HUVECs treated with HPPE (5 μM; n = 3). D, E Relative number of EdU+ cells among HUVECs treated with HPPE (5 μM; n = 3). F, G Wound healing assay of HUVECs treated with HPPE (5 μM) 24 h after challenge (n = 3). One-way analysis of variance (ANOVA) followed by Tukey’s post-hoc analysis; ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001

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