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Fig. 4 | Molecular Medicine

Fig. 4

From: STMN1–IGFBP5 axis induces senescence and extracellular matrix degradation in nucleus pulposus cells: In vivo and in vitro insights

Fig. 4

Effects of STMN1 knockdown and overexpression on NP cell senescence and ECM. A Green fluorescence intensity after lentiviral STMN1 knockdown (LV-shSTMN1) and overexpression (LV-STMN1). B Western blot analysis of STMN1 protein expression after LV-shSTMN1 and LV-STMN1 transfection into NP cells. C–D Statistical analysis of western blotting in (B) (n = 3). E Western blot analysis showing p16, p21, Aggrecan, Collagen II, ADAMTS4, MMP3, and STMN1 protein expression in different intervention groups. F NP cell SA-β-gal staining. G–H Quantitative analysis of SA-β-gal staining in (F) (n = 3). I–J Statistical analysis of western blotting in (E) (n = 3). K Immunofluorescence analysis of STMN1, p16, Collagen II, and MMP3 in different intervention groups. L–M Statistical analysis of immunofluorescence in (K) (n = 3). *, **, and *** indicate P < 0.05, P < 0.01, and P < 0.001, respectively

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Molecular Medicine

ISSN: 1528-3658