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Fig. 5 | Molecular Medicine

Fig. 5

From: STMN1–IGFBP5 axis induces senescence and extracellular matrix degradation in nucleus pulposus cells: In vivo and in vitro insights

Fig. 5

IGFBP5 as a key pro-senescence gene downstream of STMN1. A Sample extraction and PCR-Array data processing procedures for different interventions. B Heatmap of 90 cellular senescence-related genes in LV-shSTMN1 and LV-STMN1 groups. C Volcano plot showing differentially expressed genes of the LV-shSTMN1 group with 29 differentially expressed genes (Up: 2, Down: 27); volcano plot of the LV-STMN1 group with 25 differentially expressed genes (Up: 22, Down: 3), based on the criteria |log2 FC|≥ 1 and p < 0.05. D Venn diagram cross-tabulation analysis identifying 11 key genes involved in senescence. E Immunohistochemical analysis of IGFBP5 expression in human degenerative and naturally aged rat NP tissues. F Statistical analysis of immunohistochemistry (n = 3). G Western blot analysis showing STMN1 protein expression in different intervention groups. H Statistical analysis of Western blotting (n = 3). *, **, and *** indicate P < 0.05, P < 0.01, and P < 0.001, respectively

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Molecular Medicine

ISSN: 1528-3658