Fig. 6

Effects of IGFBP5 knockdown and overexpression on NP cell senescence and ECM degradation (A) Western blot analysis showing STMN1 protein expression after si-IGFBP5 and OE-IGFBP5 transfection in NP cells. B–C Statistical analysis of western blotting in (A) (n = 3). D Western blot analysis showing p16, p21, Aggrecan, Collagen II, ADAMTS4, MMP3, and STMN1 protein expression in different intervention groups. E NP cell SA-β-gal staining. F–G Quantitative analysis of SA-β-gal staining in (E) (n = 3). H Immunofluorescence analysis of STMN1, p16, Collagen II, and MMP3 in different intervention groups. I–J Statistical analysis of western blotting in (D) (n = 3). K–L Histograms of statistical analysis in (H) (n = 3). *, **, and *** indicate P < 0.05, P < 0.01, and P < 0.001, respectively