Fig. 5
From: Correction: NEK8 promotes the progression of gastric cancer by reprogramming asparagine metabolism
ASNS serves as a downstream molecule of NEK8. A IP-MS analysis revealed a significant correlation between NEK8 and ASNS. B, C ASNS levels were significantly elevated in GC cells compared to GES-1 cells. D Immunohistochemical analysis of a large-sample tissue microarray showed that ASNS was markedly overexpressed in GC tissues. E Kaplan–Meier survival analysis indicated that high ASNS expression is associated with a worse prognosis in patients with GC. F, G Pearson correlation analysis demonstrated a positive relationship between NEK8 expression and ASNS IHC staining. H, I IF and Western blot analyses confirmed that NEK8 significantly regulates ASNS protein expression. J The inhibition of ASNS protein expression by NEK8 knockdown was reversed by treatment with MG132. K NEK8 protein was pulled down using specific antibody and then ASNS protein was detected in the immunoprecipitated protein lysate of NEK8. L Subsequently, ASNS was also pulled down, and NEK8 could also be detected in the immunoprecipitated protein lysate of ASNS. *P < 0.05, **P < 0.01, ***P < 0.001