Fig. 1
From: Endogenous Galectin-8 protects against Th17 infiltration and fibrosis following acute kidney injury
Gal-8 is expressed in the kidney and its levels decrease following AKI. A β-galactosidase staining in kidneys from Lgals8−/− mice, reflecting endogenous Gal-8 expression. The inset shows a magnified view of the renal cortex. g = glomeruli, t = tubule, v = blood vessel. Scale bar: 100 μm. B Gal-8 transcript levels in the renal medulla and cortex of Lgals8+/+ mice, measured by RT-qPCR and normalized to β-actin. C Lectin staining of kidney structures in Lgals8−/− mice: collecting ducts (DBA, red), proximal tubules (LTA, green), and whole kidney (WGA, magenta). β-galactosidase staining is shown in grayscale. Arrows indicate colocalization of lectin and β-galactosidase staining. Scale bar: 50 μm. D β-galactosidase staining in kidneys from Lgals8−/− mice at 2 and 14 days after AKI induction. Scale bar: 50 μm. E Gal-8 transcript levels in kidneys from Lgals8+/+ mice at 2 and 14 days after AKI induction, assessed by RT-qPCR and normalized to β-actin. F Gal-8 transcript levels in the renal medulla and cortex of Lgals8+/+ mice at 2 days post-AKI induction, measured by RT-qPCR and normalized to β-actin. Data is presented as mean ± SEM of n = 3–9. *p < 0.05, **p < 0.01