Fig. 5
From: Endogenous Galectin-8 protects against Th17 infiltration and fibrosis following acute kidney injury
Similar fibroblast activation in the fibrotic phase in Lgal8+/+ and Lgals8−/− mice. (A) Vimentin and α-SMA protein levels compared by immunoblot 14 days after AKI induction in Lgals8+/+ and Lgals8−/− mice, normalized to GAPDH. (B) and (C) graphs quantifying the relative levels of vimentin and α-SMA, respectively. D Immunofluorescence (IF) and immunohistochemistry (IHQ) representative images of α-SMA localization in the kidney of Lgals8+/+ and Lgals8−/− mice. Scale bar: 50 μm. E Quantification of fluorescence intensity in α-SMA IF staining. F Cell proliferation activity shown by Ki67 IF and IHQ staining. G Quantification of total Ki67-positive nuclei. H Ki67-positive cells in tubular epithelial and (I) interstitial cells of Lgals8+/+ and Lgals8−/− mice. Data is presented as mean ± SEM of n = 4–6. *p < 0.05, **p < 0.01, ***p < 0.001