Fig.7
From: Endogenous Galectin-8 protects against Th17 infiltration and fibrosis following acute kidney injury
Enhanced Th17 kidney infiltration in Lgals8−/− mice. A A gating strategy was used to analyze cytokine production on stimulated CD4 and CD8 T cells from kidneys. Lymphocytes were gated based on size and granularity, and then doublet exclusion was performed. Events from these gates were plotted against Zombie Aqua viability dye and CD45 marker. Cells were further selected for CD45 and TCRβ positivity before CD4 and CD8 gating was applied. Finally, IFNγ and IL-17 production were evaluated in CD4+ and CD8+ populations. Fourteen days after damage induction, mononuclear cells from kidneys were isolated and analyzed by flow cytometry to detect the infiltration of (A) total T cells, (B) CD4+ T cells, (C) Th1 cells, (D) Th17 cells, (E) CD8+ T cells, (F) Tc1 cells and (G) Tc17 cells in Lgals8+/+ and Lgals8−/− mice. Mean ± SEM of n = 4–5. *p < 0.05, **p < 0.005