Fig. 1

Light exposure (LE) induced the expression of LCN2 and ferroptosis-related proteins in 661 W photoreceptor cells. A Cell viability, at 6, 12, and 24 h after LE (6000 lx for 5 h). B, C Western blotting and quantitative analysis of LCN2 protein expression at 6, 12, and 24 h after LE. The protein expression levels of LCN2 were normalized to those of β-actin and are presented as fold changes. D‒F Western blotting and quantitative analysis of the protein expression of SLC7A11 and GPX4 at 6, 12, and 24 h after LE. The protein expression levels of SLC7A11 and GPX4 were normalized to those of β-actin and are presented as fold changes. n = 3 per group. *P < 0.05, **P < 0.01. One-way ANOVA followed by Tukey’s post hoc test