Fig. 5

LCN2 knockdown alleviated ferroptosis and JNK pathway activation in the neural retina in vivo. A Timeline of the experimental design. B, C Western blotting and quantitative analysis of neural retinal LCN2 protein expression at 1, 3, and 7 days after light exposure (LE). The protein expression levels of LCN2 were normalized to those of β-actin and are presented as fold changes. D, E Western blotting and quantitative analysis of LCN2 protein expression in the neural retinas of rats following transduction with different AAV-shLCN2 sequences. The protein expression levels of LCN2 were normalized to those of β-actin and are presented as fold changes. F MDA levels in the indicated groups at 3 days after LE. G GSH levels in the indicated groups at 3 days after LE. H Intracellular Fe²⁺ levels in the indicated groups at 3 days after LE. I‒M Western blotting and quantitative analysis, showing that LCN2 knockdown inhibited the LE-induced increase in SLC7A11 and GPX4 and JNK phosphorylation at 3 days after LE. The protein expression levels of p-JNK were normalized to those of JNK and are presented as fold changes. The protein expression levels of JNK, SLC7A11, and GPX4 were normalized to those of β-actin and are presented as fold changes. n = 3 per group. *P < 0.05, **P < 0.01. One-way ANOVA followed by Tukey’s post hoc test